Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Localization of deformed wing virus (DWV) in the brains of the honeybee, Apis mellifera Linnaeus | Virology Journal BioMed Central Research Localization of deformed wing virus DWV in the brains of the honeybee Apis mellifera Linnaeus Karan S Shah1 Elizabeth C Evans1 2 and Marie C Pizzorno 1 3 Open Access Address Department of Biology Bucknell University Lewisburg PA 17837 USA 2Animal Behavior Program Bucknell University Lewisburg PA 17837 USA and 3Cell Biology and Biochemistry Program Bucknell University Lewisburg PA 17837 USA Email Karan S Shah - Elizabeth C Evans - Marie C Pizzorno - pizzorno@ Corresponding author Published 30 October 2009 Virology Journal 2009 6 182 doi I743-422X-6-I82 Received 11 June 2009 Accepted 30 October 2009 This article is available from http content 6 1 182 2009 Shah et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Deformed wing virus DWV is a positive-strand RNA virus that infects European honeybees Apis mellifera L. and has been isolated from the brains of aggressive bees in Japan. DWV is known to be transmitted both vertically and horizontally between bees in a colony and can lead to both symptomatic and asymptomatic infections in bees. In environmentally stressful conditions DWV can contribute to the demise of a honeybee colony. The purpose of the current study is to identify regions within the brains of honeybees where DWV replicates using in-situ hybridization. Results In-situ hybridizations were conducted with both sense and antisense probes on the brains of honeybees that were positive for DWV as measured by real-time RT-PCR. The visual neuropils demonstrated detectable levels of the DWV positive-strand genome. The mushroom bodies and antenna lobe neuropils also showed
