Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Molecular characterization and phylogenetic analysis of the complete genome of a porcine sapovirus from Chinese swine | Virology Journal BioMed Central Open Access Molecular characterization and phylogenetic analysis of the complete genome of a porcine sapovirus from Chinese swine Shixing Yang11 Wen Zhangt2 Quan Shen1 Fen Huang1 Yan Wang1 Jianguo Zhu1 Li Cui1 Zhibiao Yang1 and Xiuguo Hua 1 Address 1School of Agriculture and Biology Shanghai Jiao Tong University 800 Dong Chuan Road Shanghai 200240 PR China and 2School of Medical Science and Laboratory Medicine Jiangsu University 301 Xuefu Road Zhenjiang Jiangsu 212013 PR China Email Shixing Yang - yangshixing@ Wen Zhang - z0216wen@ Quan Shen - shenquanfly@ Fen Huang - huangfen6789@ Yan Wang - catcatmy@ Jianguo Zhu - jgzhu@ Li Cui - lcui@ Zhibiao Yang - zbyang@ Xiuguo Hua - hxg@ Corresponding author tEqual contributors Published 6 December 2009 Received 18 August 2009 Accepted 6 December 2009 Virology Journal 2009 6 216 doi l743-422X-6-2l 6 This article is available from http content 6 l 2l6 2009 Yang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Porcine sapovirus was first identified in the United States in 1980 hitherto several Asian countries have detected this virus. In 2008 the first outbreak of gastroenteritis in piglets caused by porcine sapovirus in China was reported. The complete genome of the identified SaV strain Ch-sw-savl was sequenced and analyzed to provide gene profile for this outbreak. Methods The whole genome of Ch-sw-savl was amplified by RT-PCR and was sequenced. Sequence alignment of the complete genome or RNA dependent RNA polymerase RdRp gene was done. 3 end of ORF2 with 2l-nt nucleotide insertion was further analyzed using .
