Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Evidence for a novel coding sequence overlapping the 5'-terminal ~90 codons of the Gill-associated and Yellow head okavirus envelope glycoprotein gene | Virology Journal BioMed Central Open Access Short report Evidence for a novel coding sequence overlapping the 5 -terminal 90 codons of the Gill-associated and Yellow head okavirus envelope glycoprotein gene Andrew E Firth 1 and John F Atkins 1 2 Address 1BioSciences Institute University College Cork Cork Ireland and 2Department of Human Genetics University of Utah Salt Lake City UT 84112-5330 USA Email Andrew E Firth - John F Atkins - Corresponding authors Published 17 December 2009 Received I October 2009 Accepted 17 December 2009 Virology Journal 2009 6 222 doi 1743-422X-6-222 This article is available from http content 6 1 222 2009 Firth and Atkins licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The genus Okavirus order Nidovirales includes a number of viruses that infect crustaceans causing major losses in the shrimp industry. These viruses have a linear positive-sense ssRNA genome of 26-27 kb encoding a large replicase polyprotein that is expressed from the genomic RNA and several additional proteins that are expressed from a nested set of 3 -coterminal subgenomic RNAs. In this brief report we describe the bioinformatic discovery of a new apparently coding ORF that overlaps the 5 end of the envelope glycoprotein encoding sequence ORF3 in the 2 reading frame. The new ORF has a strong coding signature and in fact is more conserved at the amino acid level than the overlapping region of ORF3. We propose that translation of the new ORF initiates at a conserved AUG codon separated by just 2 nt from the ORF3 AUG initiation codon resulting in a novel 86 amino acid protein. Findings The
