A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification | JinLong et al. Virology Journal 2010 7 14 http content 7 1 14 VIROLOGY JOURNAL RESEARCH Open Access A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification Yang JinLong1 2 Yang Rui1 2 Cheng AnChun2 3 Wang MingShu2 3 Fu LiZhi1 Yang SongQuan1 Zhang SuHui1 Yang Liu1 Xu ZhiYong4 Abstract Background Goose parvovirus GPV is a Dependovirus associated with latent infection and mortality in geese. Currently in a worldwide scale GPV severely affects geese production. The objective of this study is to develop a loop-mediated isothermal amplification LAMP method for the sensitive rapid and inexpensive detection of GPV in the field. Results A set of six specific primers was designed by targeting the GPVVP3 DNA. With Bst DNA polymerase large fragment the target DNA could be amplified at 65 C as early as 20 min of incubation in a simple water bath. A positive reaction was identified through the detection of the LAMP product by color change visible to the naked eye. The detection limit of the assay was 28 copies gl of plasmid pVP3 and with equal sensitivity and specificity to fluorescent quantitative real-time PCR FQ-PCR . Conclusions The high sensitivity specificity and simplicity as well as the high throughput make this method suitable for specific detection of GPV infection in both field conditions and laboratory settings. The utilization of complicated equipment and conduct of technical training on the GPV LAMP were not necessary. Background Goose parvovirus GPV is a well known causative agent of Gosling plague GP an acute contagious and fatal disease referred to as Derzsy s disease 1 . GPV has been formally classified as a member of the genus Dependo-virus under the family Parvoviridae 2 . It was first described as a clinical entity by Fang 3 . In the realm of research GPV has attracted much attention owing to tremendous economic loss for countries engaged in industrialized goose production the virus
