Complete genome sequence of human astrovirus genotype 6 | Guo et al. Virology Journal 2010 7 29 http content 7 1 29 VIROLOGY JOURNAL RESEARCH Open Access Complete genome sequence of human astrovirus genotype 6 Li Guo 1 Richard Gonzalez1 2 Wei Wang1 Yongjun Li1 2 Gláucia Paranhos-Baccalà2 Guy Vernet2 Jianwei Wang1 Abstract Background Human astroviruses HAstVs are one of the important causes of acute gastroenteritis in children. Currently eight HAstV genotypes have been identified and all but two HAstV-6 and HAstV-7 have been fully sequenced. We here sequenced and analyzed the complete genome of a HAstV-6 strain 192-BJ07 which was identified in Beijing China. Results The genome of 192-BJ07 consists of 6745 nucleotides. The 192-BJ07 strain displays a nucleotide sequence identity with other HAstV genotypes and exhibits amino acid sequence identities of and in the ORF1a ORF1b and ORF2 regions respectively. Homological analysis of ORF2 shows that 192-BJ07 is identical to the documented HAstV-6 strain. Further phylogenetic analysis indicates that different genomic regions are likely undergoing different evolutionary and selective pressures. No recombination event was observed in HAstV-6 in this study. Conclusion The completely sequenced and characterized genome of HAstV-6 192-BJ07 provides further insight into the genetics of astroviruses and aids in the surveillance and control of HAstV gastroenteritis. Background Human astroviruses HAstVs are one of the most common causes of acute gastroenteritis in children worldwide 1-3 . HAstV was first identified during an outbreak of gastroenteritis among hospitalized infants in 1975 4 . Its name is derived from its distinctive starshaped appearance under the electron microscopy EM . Molecular analyses indicate that HAstVs are non-envel-oped viruses with a 6-8 kb single-stranded positivesense RNA genome consisting of three overlapping open reading frames ORFs -ORF1a ORF1b and ORF2-as well as the 5 - and 3 nontranslated regions
