Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate | Wang et al. Virology Journal 2010 7 34 http content 7 1 34 VIROLOGY JOURNAL RESEARCH Open Access Expression purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate Ruoyu Wang 1 Guangpeng Wang2 Qi Zhao3 Yu Zhang3 Lizhe An 1 3 and Yun Wang 4 Abstract Background Lily symptomless virus LSV is widespread in many countries where lily are grown or planted and causes severe economic losses in terms of quantity and quality of flower and bulb production. To study the structure-function relationship of coat protein CP of LSV to investigate antigenic relationships between coat protein subunits or intact virons and to prepare specific antibodies against LSV substantial amounts of CP protein are needed. Results Thus full-length cDNA of LSV coat protein was synthesized and amplified by RT-PCR from RNA isolated from LSV Lanzhou isolate. The extended kDa CP was cloned and expressed prokaryoticly and then purified by Ni-ion affinity chromatography. Its identity and antigenicity of recombinant CP were identified on Western-blotting by using the prepared anti-LSV antibodies. Conclusions The results indicate that fusion CP maintains its native antigenicity and specificity providing a good source of antigen in preparation of LSV related antibodies. Detailed structural analysis of a pure recombinant CP should allow a better understanding of its role in cell attachment and LSV tropism. This investigation to LSV should provide some specific antibodies and aid to development a detection system for LSV diagnostics and epidemiologic surveys. Background Lanzhou lily L. davidii is an important bulb edible crop which mostly distributes in middle area of Gansu province in China. Virus infection caused serious reduction in production of Lanzhou lily and other economic corps in recent years 1 2 . Lily symptomless virus LSV family Genus Carlavirus species is the most prevalent virus infecting Lanzhou lily 2 and it has been .
