Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The directionality of the nuclear transport of the influenza A genome is driven by selective exposure of nuclear localization sequences on nucleoprotein | Virology Journal BioMed Central Research The directionality of the nuclear transport of the influenza A genome is driven by selective exposure of nuclear localization sequences on nucleoprotein Winco WH Wu and Nelly Panté Open Access Address Department of Zoology University of British Columbia 6270 University Boulevard Vancouver British Columbia V6T 1Z4 Canada Email Winco WH Wu - winco@ Nelly Panté - pante@ Corresponding author Published 2 June 2009 Received 9 April 2009 Virology Journal 2009 6 68 doi 1743-422X-6-68 Accepted 2 June 2009 This article is available from http content 6 1 68 2009 Wu and Panté licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Early in infection the genome of the influenza A virus consisting of eight complexes of RNA and proteins termed viral ribonucleoproteins vRNPs enters the nucleus of infected cells for replication. Incoming vRNPs are imported into the nucleus of infected cells using at least two nuclear localization sequences on nucleoprotein NP NLS1 at the N terminus and NLS2 in the middle of the protein . Progeny vRNP assembly occurs in the nucleus and later in infection these are exported from the nucleus to the cytoplasm. Nuclear-exported vRNPs are different from incoming vRNPs in that they are prevented from re-entering the nucleus. Why nuclear-exported vRNPs do not re-enter the nucleus is unknown. Results To test our hypothesis that the exposure of NLSs on the vRNP regulates the directionality of the nuclear transport of the influenza vRNPs we immunolabeled the two NLSs of NP NLS1 and NLS2 and analyzed their surface accessibility in cells infected with the influenza A virus. We found that the NLS1 epitope on NP was
