Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The role of NH4Cl and cysteine proteases in Human Papillomavirus type 16 infection | Virology Journal BioMed Central Research The role of NH4Cl and cysteine proteases in Human Papillomavirus type 16 infection Sarah A Dabydeen 1 and Patricio I Meneses 2 Address Department of Microbiology and Immunology . Bligh Cancer Research Laboratory School of Graduate and Postdoctoral Studies Rosalind Franklin University of Medicine and Science North Chicago IL USA and 2Department of Microbiology and Immunology . Bligh Cancer Research Laboratory Chicago Medical School Rosalind Franklin University of Medicine and Science North Chicago IL USA Email Sarah A Dabydeen - Patricio I Meneses - Corresponding author fEqual contributors Open Access Published 20 July 2009 Received 20 April 2009 Virology Journal 2009 6 109 doi 1743-422X-6-109 Accepted 20 July 2009 This article is available from http content 6 1 109 2009 Dabydeen and Meneses licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The infectious pathway of the non-enveloped Human Papillomavirus Type 16 HPV16 includes binding to the cell surface clathrin-mediated endocytosis and penetration into an endosome. HPV16 infection was shown to decrease in the presence of the lysosomotrophic neutralizing agent ammonium chloride NH4Cl . NH4Cl neutralizes acidic endo-lysosome compartments thus suggesting that pH was responsible for PV capsid conformational changes leading endosome escape. Results However our data suggested that NH4Cl blocked infection by preventing the movement of PV viral particles from the early endosome to the caveosome as was shown for JC virus 1 2 . We have confirmed that HPV 16 infection requires the trafficking of reporter-virions to the caveosome as
