Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Effects of vaccinia virus uracil DNA glycosylase catalytic site and deoxyuridine triphosphatase deletion mutations individually and together on replication in active and quiescent cells and pathogenesis in mice | Virology Journal BioMed Central Research Effects of vaccinia virus uracil DNA glycosylase catalytic site and deoxyuridine triphosphatase deletion mutations individually and together on replication in active and quiescent cells and pathogenesis in mice Frank S De Silva1 2 and Bernard Moss 1 Open Access Address laboratory of Viral Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda Maryland 20892-3210 USA and Scientific Review Program NIAID NIH 6700B Rockledge Dr. Bethesda MD 20892-7616 USA Email Frank S De Silva - fdesilva@ Bernard Moss - bmoss@ Corresponding author Published 2 December 2008 Received 25 November 2008 Accepted 2 December 2008 Virologyjournal 2008 5 145 doi 1743-422X-5-145 This article is available from http content 5 1 145 2008 De Silva and Moss licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Low levels of uracil in DNA result from misincorporation of dUMP or cytosine deamination. Vaccinia virus VACV the prototype poxvirus encodes two enzymes that can potentially reduce the amount of uracil in DNA. Deoxyuridine triphosphatase dUTPase hydrolyzes dUTP generating dUMP for biosynthesis of thymidine nucleotides while decreasing the availability of dUTP for misincorporation uracil DNA glycosylase UNG cleaves uracil N-glycosylic bonds in DNA initiating base excision repair. Studies with actively dividing cells showed that the VACV UNG protein is required for DNA replication but the UNG catalytic site is not whereas the dUTPase gene can be deleted without impairing virus replication. Recombinant VACV with an UNG catalytic site mutation was attenuated in vivo while a dUTPase deletion mutant was not. .
