Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A simple and rapid Hepatitis A Virus (HAV) titration assay based on antibiotic resistance of infected cells: evaluation of the HAV neutralization potency of human immune globulin preparations | BioMed Central Virology Journal Open Access Methodology A simple and rapid Hepatitis A Virus HAV titration assay based on antibiotic resistance of infected cells evaluation of the HAV neutralization potency of human immune globulin preparations Krishnamurthy Konduru1 Maria Luisa Virata-Theimer2 Mei-ying W Yu2 and Gerardo G Kaplan 1 Address Division of Emerging and Transfusion Transmitted Diseases Center for Biologics Evaluation and Research Food and Drug Administration 29 Lincoln Drive Bethesda Maryland 20892 USA and 2Division of Hematology Center for Biologics Evaluation and Research Food and Drug Administration 29 Lincoln Drive Bethesda Maryland 20892 USA Email Krishnamurthy Konduru - Maria Luisa Virata-Theimer - Mei-ying W Yu - Gerardo G Kaplan - gk@ Corresponding author Published 18 December 2008 Received 5 December 2008 Accepted 18 December 2008 Virology Journal 2008 5 155 doi l743-422X-5-l55 This article is available from http content 5 l l55 2008 Konduru et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hepatitis A virus HAV the causative agent of acute hepatitis in humans is an atypical Picornaviridae that grows poorly in cell culture. HAV titrations are laborious and timeconsuming because the virus in general does not cause cytopathic effect and is detected by immunochemical or molecular probes. Simple HAV titration assays could be developed using currently available viral construct containing selectable markers. Results We developed an antibiotic resistance titration assay ARTA based on the infection of human hepatoma cells with a wild type HAV construct containing
