Detection of novel insect flavivirus sequences integrated in Aedes albopictus (Diptera: Culicidae) in Northern Italy | Virology Journal BioMed Central Open Access Short report Detection of novel insect flavivirus sequences integrated in Aedes albopictus Diptera Culicidae in Northern Italy David Roiz 1 Ana Vazquez2 Mari Paz Sanchez Seco2 Antonio Tenorio2 and Annapaola Rizzoli1 Address 1IASMA Research and Innovation Centre Environment and Natural Resources Area Edmund Mach Foundation S. Michele all Adige TN Italy and 2Laboratorio de arbovirus y enfermedades víricas importadas. Centro Nacional de Microbiologia Instituto de Salud Carlos III Madrid Spain Email David Roiz - davidroiz@ Ana Vázquez - Mari Paz Sánchez Seco - Antonio Tenorio - atenorio@ Annapaola Rizzoli - rizzoli@ Corresponding author Published 5 July 2009 Received 19 May 2009 Virology Journal 2009 6 93 doi 186 1743-422X-6-93 Accepted 5 July 2009 This article is available from http content 6 1 93 2009 Roiz et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ The presence of DNA sequences integrated from a new flavivirus related to Cell Fusing Agent and Kamiti River Virus was identified in wild Aedes albopictus mosquito populations from the provinces of Trentino and Padova Northern Italy. Field work was developed during August-October 2007 with BG-traps and mosquitoes were screened for flavivirus and alphavirus. No alphavirus was detected indicating that Chikungunya virus is not present in these mosquitoes in Trentino and Padova area. However 21 of the pools were positive for flavivirus further recognised with BLAST as similar to Kamiti River Virus. Phylogenetical analysis with 708 nucleotides from the NS5 gene .
