Tham khảo luận văn - đề án 'báo cáo khoa học: " improved efficacy of a gene optimised adenovirus-based vaccine for venezuelan equine encephalitis virus"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Improved Efficacy of a Gene Optimised Adenovirus-based Vaccine for Venezuelan Equine Encephalitis Virus Amanda J Williams Lyn M O Brien Robert J Phillpotts and Stuart D Perkins Open Access Address Biomedical Sciences Department Defence Science and Technology Laboratory Porton Down Salisbury Wiltshire SP4 OJQ UK Email Amanda J Williams - ajwilliams@ Lyn M O Brien - lmobrien@ Robert J Phillpotts - bjphillpotts@ Stuart D Perkins - sdperkins@ Corresponding author Published 31 July 2009 Received 30 March 2009 Virology Journal 2009 6 118 doi 1743-422X-6-118 Accepted 31 July 2009 This article is available from http content 6 1 1 18 2009 Crown Copyright Dstl Abstract Background Optimisation of genes has been shown to be beneficial for expression of proteins in a range of applications. Optimisation has increased protein expression levels through improved codon usage of the genes and an increase in levels of messenger RNA. We have applied this to an adenovirus ad -based vaccine encoding structural proteins E3-E2-6K of Venezuelan equine encephalitis virus VEEV . Results Following administration of this vaccine to Balb c mice an approximately ten-fold increase in antibody response was elicited and increased protective efficacy compared to an ad-based vaccine containing non-optimised genes was observed after challenge. Conclusion This study in which the utility of optimising genes encoding the structural proteins of VEEV is demonstrated for the first time informs us that including optimised genes in gene-based vaccines for VEEV is essential to obtain maximum immunogenicity and protective efficacy. Background Venezuelan equine encephalitis virus VEEV is a positive-stranded enveloped RNA virus of the genus Alphavirus in the family Togaviridae. VEEV causes a disease in humans characterized by fever headache and occasionally encephalitis. It is the cause of recent .
