Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.) | BMC Plant Biology BioMed Central Research article Quantification of the tissue-culture induced variation in barley Hordeum vulgare L. Piotr TBednarek t1 Renata Orlowskat2 Robert MD Koebnert3 and Janusz Zimnyt1 Open Access Address 1Plant Breeding Acclimatization Institute 05-870 Blonie Radzikow Poland 2UWM Olsztyn Poland and 3John Innes Centre Colney Lane Norwich NR4 7UH UK Email PiotrT Bednarek - Renata Robert MD Koebner - mockbeggars@ Janusz Zimny - Corresponding author tEqual contributors Published 2 March 2007 Received I August 2006 BMC Plant Biology 2007 7 10 doi 1471-2229-7-10 Accepted 2 March 2007 This article is available from http 1471-2229 7 10 2007 Bednarek et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background When plant tissue is passaged through in vitro culture many regenerated plants appear to be no longer clonal copies of their donor genotype. Among the factors that affect this so-called tissue culture induced variation are explant genotype explant tissue origin medium composition and the length of time in culture. Variation is understood to be generated via a combination of genetic and or epigenetic changes. A lack of any phenotypic variation between regenerants does not necessarily imply a concomitant lack of genetic or epigenetic change and it is therefore of interest to assay the outcomes of tissue culture at the genotypic level. Results A variant of methylation sensitive AFLP based on the isoschizomeric combinations Acc65I MseI and KpnI MseI was applied to analyze at both the sequence and methylation levels the outcomes of regeneration from tissue culture in barley. .
