Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of gene expression in cotton fiber initials | BMC Plant Biology BioMed Central Research article Analysis of gene expression in cotton fiber initials Earl WTaliercio 1 and Deborah Boykin2 Open Access Address 1USDA ARS 3127 Ligon St Raleigh NC 27607 USA and 2USDA ARS 141 Experiment Station Rd. Stoneville MS 38776 USA Email Earl W Taliercio - Deborah Boykin - dboykin@ Corresponding author Published 16 May 2007 Received 25 September 2006 BMC Plant Biology 2007 7 22 doi l47l-2229-7-22 Accepted 16 May 2007 This article is available from http l47l-2229 7 22 2007 Taliercio and Boykin licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Cotton Gossypium hirsutum L. fibers are trichomes that initiate from the ovule epidermis. Little is known about the developmental pathway causing fiber to differentiate from ovular epidermal cells even though limits on the number of cells that differentiate into fiber will limit yield. Results A method was developed to isolate RNA from fiber initials l day post anthesis dpa . Complementary DNA libraries representing l dpa fibers and other cotton tissues were sequenced and analyzed. Assembly of G. hirsutum Expressed Sequenced Tags ESTs identified over ll 000 sequences not previously represented in GenBank. New genes identified among these ESTs were represented on microarrays. The microarrays were used to identify genes enriched in fiber initials l dpa fibers and elongating fibers. Analyses of Gene Ontologies GO of differentially expressed genes determined that terms associated with the membranes were statistically over represented among genes increased in expression in fiber initials and l0 dpa fibers. Staining ovules with a fluorescent dye .
