Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The red fluorescent protein eqFP611: application in subcellular localization studies in higher plants | BMC Plant Biology BioMed Central Research article Open Access The red fluorescent protein eqFP6ll application in subcellular localization studies in higher plants Joachim Forner and Stefan Binder Address Molekulare Botanik Universitat Ulm Albert-Einstein-Allee 11 89069 Ulm Germany Email Joachim Forner - Stefan Binder - Corresponding author Published 6 June 2007 Received 8 November 2006 BMC Plant Biology 2007 7 28 doi 1471-2229-7-28 Accepted 6 June 2007 This article is available from http 1471-2229 7 28 2007 Forner and Binder licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Intrinsically fluorescent proteins have revolutionized studies in molecular cell biology. The parallel application of these proteins in dual- or multilabeling experiments such as subcellular localization studies requires non-overlapping emission spectra for unambiguous detection of each label. In the red spectral range almost exclusively DsRed and derivatives thereof are used today. To test the suitability of the red fluorescent protein eqFP611 as an alternative in higher plants the behavior of this protein was analyzed in terms of expression subcellular targeting and compatibility with GFP in tobacco. Results When expressed transiently in tobacco protoplasts eqFP611 accumulated over night to levels easily detectable by fluorescence microscopy. The native protein was found in the nucleus and in the cytosol and no detrimental effects on cell viability were observed. When fused to N-terminal mitochondrial and peroxisomal targeting sequences the red fluorescence was located exclusively in the corresponding organelles in transfected protoplasts. Upon .
