Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines | BMC Plant Biology BioMed Central Research article Open Access Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines Simone D Castellarin1 2 and Gabriele Di Gaspero 1 2 Address 1Istituto di Genomica Applicata Parco Scientifico e Tecnologico Luigi Danieli via Jacopo Linussio 51 33100 Udine Italy and 2Dipartimento di Scienze Agrarie e Ambientali University of Udine via delle Scienze 208 33100 Udine Italy Email Simone D Castellarin - Gabriele Di Gaspero - Corresponding author Published 30 August 2007 Received 30 March 2007 BMC Plant Biology 2007 7 46 doi 1471-2229-7-46 Accepted 30 August 2007 This article is available from http 1471-2229 7 46 2007 Castellarin and Di Gaspero licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Fruit coloration of red-skinned grapevines is mainly due to anthocyanin pigments. We analysed a panel of nine cultivars that included extreme phenotypes for berry colour ranging from green absence of anthocyanins to red purple violet and blue. Expression of six genes of the anthocyanin pathway coding for flavanone-hydroxylase F3H flavonoid 3 -hydroxylase F3 H flavonoid 3 5 -hydroxylase F3 5 H UDP-glucose flavonoid-3-0-glucosyltransferase UFGT glutathione-S-transferase GST O-methyltransferase OMT and four transcription factors MybA MybB MybC MybD was analysed by quantitative RT-PCR at four developmental stages from before the onset of ripening until full maturity and compared to anthocyanin metabolites. Results Total anthocyanin content at full maturity correlated well with the cumulative expression of F3H UFGT and GST .
