Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum | BMC Plant Biology BioMed Central Research article Open Access Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum Gunnhild W Takle1 2 Ian K Toth3 and May B Brurberg 1 Address Norwegian Institute for Agricultural and Environmental Research Plant Health and Plant Protection Division H0gskoleveien 7 1432 Ảs Norway 2Norwegian University of Life Sciences Institute for Chemistry Biotechnology and Food Science PO Box 5003 1432 Ảs Norway and 3SCRI Invergowrie Dundee DD2 5DA UK Email Gunnhild WTakle - Ian K Toth - May B Brurberg - Corresponding author Published 21 September 2007 Received 13 February 2007 BMC Plant Biology 2007 7 50 doi l47l-2229-7-50 Accepted 21 September 2007 This article is available from http l47l-2229 7 50 2007 Takle et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Real-time RT-PCR has become a powerful technique to monitor low-abundance mRNA expression and is a useful tool when examining bacterial gene expression inside infected host tissues. However correct evaluation of data requires accurate and reliable normalisation against internal standards. Thus the identification of reference genes whose expression does not change during the course of the experiment is of paramount importance. Here we present a study where manipulation of cultural growth conditions and in planta experiments have been used to validate the expression stability of reference gene candidates for the plant pathogen Pectobacterium atrosepticum belonging to the family Enterobacteriaceae. Results Of twelve reference gene candidates tested four proved to
