Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Cross-species EST alignments reveal novel and conserved alternative splicing events in legumes | BMC Plant Biology BioMed Central Research article Open Access Cross-species EST alignments reveal novel and conserved alternative splicing events in legumes Bing-Bing Wang1 3 Mike O Toole1 Volker Brendel2 and Nevin D Young 1 Address Department of Plant Pathology University of Minnesota St. Paul MN 55108 USA 2Department of Genetics Development and Cell Biology and Department of Statistics Iowa State University Ames IA 50011 USA and 3Pioneer Hi-Bred International Inc. a DuPont company 7200 . 62nd Avenue Johnston IA 50131 USA Email Bing-Bing Wang - wangx741@ Mike O Toole - Volker Brendel - vbrendel@ Nevin D Young - neviny@ Corresponding author Published 19 February 2008 Received 16 October 2007 BMC Plant Biology 2008 8 17 doi l47l-2229-8-l7 Accepted 19 February 2008 This article is available from http l47l-2229 8 l7 2008 Wang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Although originally thought to be less frequent in plants than in animals alternative splicing AS is now known to be widespread in plants. Here we report the characteristics of AS in legumes one of the largest and most important plant families based on EST alignments to the genome sequences of Medicago truncatula Mt and Lotus japonicus Lj . Results Based on cognate EST alignments alone the observed frequency of alternatively spliced genes is lower in Mt l0 l l07 genes and Lj 3 92 genes than in Arabidopsis and rice both around 20 . However AS frequencies are comparable in all four species if EST levels are normalized. Intron retention is the most common form of AS in all four plant species 50 with slightly lower frequency in legumes compared to .
