Xét nghiệm chẩn đoán cấp tính và mạn tính trong viêm gan C | 27 cổ đông thiểu số của bệnh nhân và không thể phân biệt đối xử giữa mãn tính và cấp tính viêm gan C. | 3. Diagnostic Tests in Acute and Chronic Hepatitis C 27 minority of patients and cannot discriminate between acute and chronic hepatitis C. False-positive results are more frequent in patients with rheuma factors and in populations with a low hepatitis C prevalence for example in blood and organ donors. False-negative HCV antibody testing may occur in patients on haemodialysis or in severely immunosuppressed patients or in haematological malignancies. One quantitative HCV core antigen assay Architect HCV Ag Abbott Diagnostics has been approved so far. This assay comprises 5 different antibodies is highly specific and shows equivalent sensitivity for determination of chronic hepatitis C as HCV RNA measurement Morota 2009 . Overall the sensitivity of the core antigen assay is lower in comparison to highly sensitive HCV RNA assays and data on the potential use of the core antigen assay instead of HCV RNA tests for management of antiviral therapy have not been presented yet. Nucleic Acid Testing for HCV Because of the importance of an exact HCV RNA load determination for therapeutic management the World Health Organization who established the HCV RNA international standard based on international units iu which is used in all clinically applied HCV RNA tests. Currently several HCV RNA assays are commercially available. Qualitative HCV RNA tests include the qualitative RT-PCR of which the Amplicor HCV Roche Molecular Systems USA is an FDA- and CE-approved RT-PCR system for qualitative HCV RNA testing that allows detection of HCV RNA concentrations down to 50 lU ml of all HCV genotypes Nolte 2001 . Transcription-mediated amplification- TMA -based qualitative HCV RNA detection has a very high sensitivity lower limit This is trial version 28 Hepatitis C Guide of detection 5-10 lU ml Sarrazin 2002 Hendricks 2003 . A commercially available TMA assay is the Versant HCV RNA Qualitative Assay Siemens Medical Solutions Diagnostics Germany . This system .
