Autologous tumor-derived heat-shock protein peptide complex-96 (HSPPC-96) in patients with metastatic melanoma | Eton et al. Journal of Translational Medicine 2010 8 9 http content 8 1 9 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Autologous tumor-derived heat-shock protein peptide complex-96 HSPPC-96 in patients with metastatic melanoma Omar Eton1 Merrick I Ross2 Mary Jo East1 Paul F Mansfield2 Nicholas Papadopoulos1 Julie A Ellerhorst3 Agop Y Bedikian1 Jeffrey E Lee2 Abstract Background Glycoprotein-96 a non-polymorphic heat-shock protein associates with intracellular peptides. Autologous tumor-derived heat shock protein-peptide complex 96 HSPPC-96 can elicit potent tumor-specific T cell responses and protective immunity in animal models. We sought to investigate the feasibility safety and antitumor activity of HSPPC-96 vaccines prepared from tumor specimens of patients with metastatic melanoma. Methods Patients with a Karnofsky Performance Status 70 and stage III or stage IV melanoma had to have a metastasis 3 cm in diameter resectable as part of routine clinical management. HSPPC-96 tumor-derived vaccines were prepared in one of three dose levels 25 or 100 pg dose and administered as an intradermal injection weekly for 4 consecutive weeks. In vivo induction of immunity was evaluated using delayed-type hypersensitivity DTH to HSPPC-96 irradiated tumor and dinitrochlorobenzene DNCB . The g-interferon IFNg ELISPOT assay was used to measure induction of a peripheral blood mononuclear cell response against autologous tumor cells at baseline and at the beginning of weeks 3 4 and 8. Results Among 36 patients enrolled 72 had stage IV melanoma and 83 had received prior systemic therapy. The smallest tumor specimen from which HSPPC-96 was prepared weighed 2 g. Twelve patients including 9 with stage IV and indicator lesions had a negative DNCB skin test result at baseline. All 36 patients were treated and evaluable for toxicity and response. There were no serious toxicities. There were no observed DTH responses to HSPPC-96 or to .
