Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Microrna profiling analysis of differences between the melanoma of young adults and older adults | Jukic et al. Journal of Translational Medicine 2010 8 27 http content 8 1 27 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Microrna profiling analysis of differences between the melanoma of young adults and older adults Drazen M Jukic1 2t Uma NM Rao2 Lori Kelly2t Jihad S Skaf3 Laura M Drogowski1 John M Kirkwood4 Monica C Panelli4 Abstract Background This study represents the first attempt to perform a profiling analysis of the intergenerational differences in the microRNAs miRNAs of primary cutaneous melanocytic neoplasms in young adult and older age groups. The data emphasize the importance of these master regulators in the transcriptional machinery of melanocytic neoplasms and suggest that differential levels of expressions of these miRs may contribute to differences in phenotypic and pathologic presentation of melanocytic neoplasms at different ages. Methods An exploratory miRNA analysis of 666 miRs by low density microRNA arrays was conducted on formalin fixed and paraffin embedded tissues FFPE from 10 older adults and 10 young adults including conventional melanoma and melanocytic neoplasms of uncertain biological significance. Age-matched benign melanocytic nevi were used as controls. Results Primary melanoma in patients greater than 60 years old was characterized by the increased expression of miRs regulating TLR-MyD88-NF-kappaB pathway hsa-miR-199a RAS RAB22A pathway hsa-miR-204 growth differentiation and migration hsa-miR337 epithelial mesenchymal transition EMT let-7b hsa-miR-10b 10b invasion and metastasis hsa-miR-10b 10b hsa-miR-30a e hsa-miR-29c cellular matrix components hsa-miR-29c invasion-cytokinesis hsa-miR-99b compared to melanoma of younger patients. MiR-211 was dramatically downregulated compared to nevi controls decreased with increasing age and was among the miRs linked to metastatic processes. Melanoma in young adult patients had increased expression of hsa-miR-449a and decreased expression of .
