Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Papillomavirus pseudovirions packaged with the L2 gene induce cross-neutralizing antibodies | Combelas et al. Journal of Translational Medicine 2010 8 28 http content 8 1 28 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Papillomavirus pseudovirions packaged with the L2 gene induce cross-neutralizing antibodies 1 1 1 1 Nicolas Combelas Emilie Saussereau Maxime JJ Fleury Tatiana Ribeiro Julien Gaitan Diego F Duarte-Forero1 2 Pierre Coursaget1 Antoine Touzé1 Abstract Background Current vaccines against HPVs are constituted of L1 protein self-assembled into virus-like particles VLPs and they have been shown to protect against natural HPV16 and HPV18 infections and associated lesions. In addition limited cross-protection has been observed against closely related types. Immunization with L2 protein in animal models has been shown to provide cross-protection against distant papillomavirus types suggesting that the L2 protein contains cross-neutralizing epitopes. However vaccination with L2 protein or L2 peptides does not induce high titers of anti-L2 antibodies. In order to develop a vaccine with the potential to protect against other high-risk HPV types we have produced HPV58 pseudovirions encoding the HPV31 L2 protein and compared their capacity to induce cross-neutralizing antibodies with that of HPV L1 and HPV L1 L2 VLPs. Methods The titers of neutralizing antibodies against HPV16 HPV18 HPV31 and HPV58 induced in Balb c mice were compared after immunization with L2-containing vaccines. Results Low titers of cross-neutralizing antibodies were detected in mice when immunized with L1 L2 VLPs and the highest levels of cross-neutralizing antibodies were observed in mice immunized with HPV 58 L1 L2 pseudovirions encoding the HPV 31 L2 protein. Conclusions The results obtained indicate that high levels of cross-neutralizing antibodies are only observed after immunization with pseudovirions encoding the L2 protein. HPV pseudovirions thus represent a possible new strategy for the generation of a broad-spectrum vaccine to .
