Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Adipose-derived mesenchymal stem cells markedly attenuate brain infarct size and improve neurological function in rats | Leu et al. Journal of Translational Medicine 2010 8 63 http content 8 1 63 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Adipose-derived mesenchymal stem cells markedly attenuate brain infarct size and improve neurological function in rats Steve Leu41 Yu-Chun Lin 1 4 Chun-Man Yuen42 Chia-Hung Yen3 Ying-Hsien Kao4 Cheuk-Kwan Sun 45 and Hon-Kan Yip 1 6 Abstract Background The therapeutic effect of adipose-derived mesenchymal stem cells ADMSCs on brain infarction area BIA and neurological status in a rat model of acute ischemic stroke IS was investigated. Methods Adult male Sprague-Dawley SD rats n 30 were divided into IS plus intra-venous 1 mL saline at 0 12 and 24 h after IS induction control group and IS plus intra-venous ADMSCs X 106 treated interval as controls treatment group after occlusion of distal left internal carotid artery. The rats were sacrificed and brain tissues were harvested on day 21 after the procedure. Results The results showed that BIA was larger in control group than in treatment group p . The sensorimotor functional test Corner test identified a higher frequency of turning movement to left in control group than in treatment group p . mRNA expressions of Bax caspase 3 interleukin IL -18 toll-like receptor-4 and plasminogen activator inhibitor-1 were higher whereas Bcl-2 and IL-8 Gro were lower in control group than in treatment group all p . Western blot demonstrated a lower CXCR4 and stromal-cell derived factor-1 SDF-1 in control group than in treatment group all p . Immunohistofluorescent staining showed lower expressions of CXCR4 SDF-1 von Willebran factor and doublecortin whereas the number of apoptotic nuclei on TUNEL assay was higher in control group than in treatment group all p . Immunohistochemical staining showed that cellular proliferation and number of small vessels were lower but glial fibrillary acid protein was higher in control group than in treatment group
