Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with early-stage breast cancer | Pietrowska et al. Journal of Translational Medicine 2010 8 66 http content 8 1 66 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with early-stage breast cancer Monika Pietrowska21 Joanna Polanska22 Lukasz Marczak3 Katarzyna Behrendt 1 Elzbieta Nowicka1 Maciej Stobiecki3 Andrzej Polanski2 4 Rafal Tarnawski1 and Piotr Widlak 1 Abstract Background The proteomics approach termed proteome pattern analysis has been shown previously to have potential in the detection and classification of breast cancer. Here we aimed to identify changes in serum proteome patterns related to therapy of breast cancer patients. Methods Blood samples were collected before the start of therapy after the surgical resection of tumors and one year after the end of therapy in a group of 70 patients diagnosed at early stages of the disease. Patients were treated with surgery either independently 26 or in combination with neoadjuvant chemotherapy 5 or adjuvant radio chemotherapy 39 . The low-molecular-weight fraction of serum proteome was examined using MALDI-ToF mass spectrometry and then changes in intensities of peptide ions registered in a mass range between 2 000 and 14 000 Da were identified and correlated with clinical data. Results We found that surgical resection of tumors did not have an immediate effect on the mass profiles of the serum proteome. On the other hand significant long-term effects were observed in serum proteome patterns one year after the end of basic treatment we found that about 20 peptides exhibited significant changes in their abundances . Moreover the significant differences were found primarily in the subgroup of patients treated with adjuvant therapy but not in the subgroup subjected only to surgery. This suggests that the observed changes reflect overall responses of the patients to the toxic effects of adjuvant radio chemotherapy.
