ETuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: pigenetic change in E-Cardherin and COX-2 to predict chronic periodontitis | Loo et al. Journal of Translational Medicine 2010 8 110 http content 8 1 110 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Epigenetic change in E-Cardherin and COX-2 to predict chronic periodontitis Wings TY Loo1 Lijian Jin1 Mary NB Cheung2 Min Wang3 Louis WC Chow4 Abstract Background DNA methylation of certain genes frequently occurs in neoplastic cells. Although the cause remains unknown many genes have been identified with such atypical methylation in neoplastic cells. The hypermethylation of E-Cadherin and Cyclooxygenase 2 COX-2 in chronic inflammation such as chronic periodontitis may demonstrate mild lesion mutation epigenetic level. This study compares the hypermethylation status of E-Cadherin and COX-2 genes which are often found in breast cancer patients with that in chronic periodontitis. Methods Total DNA was extracted from the blood samples of 108 systemically healthy non-periodontitis subjects and the gingival tissues and blood samples of 110 chronic periodontitis patient as well as neoplastic tissues of 106 breast cancer patients. Methylation-specific PCR for E-Cadherin and COX-2 was performed on these samples and the PCR products were analyzed on 2 agarose gel. Results Hypermethylation of E-Cadherin and COX-2 was observed in 38 and 35 of the breast cancer samples respectively. In chronic periodontitis patients the detection rate was 25 and 19 respectively and none was found in the systemically healthy non-periodontitis control subjects. The hypermethylation status was shown to be correlated among the three groups with statistical significance p . The methylation of CpG islands in E-Cadherin and COX-2 genes in periodontitis patients occurs more frequently in periodontitis patients than in the control subjects but occurs less frequently than in the breast cancer patients. Conclusions This set of data shows that the epigenetic change in E-Cadherin and Cyclooxygenase-2 is associated with chronic .
