Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Retention of progenitor cell phenotype in otospheres from guinea pig and mouse cochlea | Oiticica et al. Journal of Translational Medicine 2010 8 119 http content 8 1 119 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Retention of progenitor cell phenotype in otospheres from guinea pig and mouse cochlea 1 1 2 1 Jeanne Oiticica Luiz Carlos M Barboza-Junior Ana Carla Batissoco Karina Lezirovitz Regina C Mingroni-Netto2 Luciana A Haddad2 Ricardo F Bento1 Abstract Background Culturing otospheres from dissociated organ of Corti is an appropriate starting point aiming at the development of cell therapy for hair cell loss. Although guinea pigs have been widely used as an excellent experimental model for studying the biology of the inner ear the mouse cochlea has been more suitable for yielding otospheres in vitro. The aim of this study was to compare conditions and outcomes of otosphere suspension cultures from dissociated organ of Corti of either mouse or guinea pig at postnatal day three P3 and to evaluate the guinea pig as a potential cochlea donor for preclinical cell therapy. Methods Organs of Corti were surgically isolated from P3 guinea pig or mouse cochlea dissociated and cultivated under non-adherent conditions. Cultures were maintained in serum-free DMEM F12 medium supplemented with epidermal growth factor EGF plus either basic fibroblast growth factor bFGF or transforming growth factor alpha TGFa . Immunofluorescence assays were conducted for phenotype characterization. Results The TGFa group presented a number of spheres significantly higher than the bFGF group. Although mouse cultures yielded more cells per sphere than guinea pig cultures sox2 and nestin distributed similarly in otosphere cells from both organisms. We present evidence that otospheres retain properties of inner ear progenitor cells such as self-renewal proliferation and differentiation into hair cells or supporting cells. Conclusions Dissociated guinea pig cochlea produced otospheres in vitro expressing sox2 and nestin similarly to mouse .
