Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: High expression of transcriptional coactivator p300 correlates with aggressive features and poor prognosis of hepatocellular carcinoma | Li et al. Journal of Translational Medicine 2011 9 5 http content 9 1 5 TRANSLATIONAL MEDICINE RESEARCH Open Access High expression of transcriptional coactivator p300 correlates with aggressive features and poor prognosis of hepatocellular carcinoma Mei Li1 2 Rong-Zhen Luo Jie-Wei Chen1 2 Yun Cao1 2 Jia-Bin Lu1 2 Jie-Hua He1 2 Qiu-Liang Wu1 2 Mu-Yan Cai1 2 Abstract Background It has been suggested that p300 participates in the regulation of a wide range of cell biological processes and mutation of p300 has been identified in certain types of human cancers. However the expression dynamics of p300 in hepatocellular carcinoma HCC and its clinical prognostic significance are unclear. Methods In this study the methods of reverse transcription-polymerase chain reaction RT-PCR Western blotting and immunohistochemistry IHC were utilized to investigate protein mRNA expression of p300 in HCCs. Receiver operating characteristic ROC curve analysis spearman s rank correlation Kaplan-Meier plots and Cox proportional hazards regression model were used to analyze the data. Results Up-regulated expression of p300 mRNA and protein was observed in the majority of HCCs by RT-PCR and Western blotting when compared with their adjacent non-malignant liver tissues. According to the ROC curves the cutoff score for p300 high expression was defined when more than 60 of the tumor cells were positively stained. High expression of p300 was examined in 60 123 of HCCs and in 8 123 of adjacent non-malignant liver tissues. High expression of p300 was correlated with higher AFP level larger tumor size multiplicity poorer differentiation and later stage P . In univariate survival analysis a significant association between overexpression of p300 and shortened patients survival was found P . In different subsets of HCC patients p300 expression was also a prognostic indicator in patients with stage II P and stage III P . Importantly p300 .
