Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Porcine adenovirus type 3 E1Blarge protein downregulates the induction of IL-8 | Virology Journal BioMed Central Research Porcine adenovirus type 3 E1Blarge protein downregulates the induction of IL-8 Yan Zhou Andrew Ficzycz and Suresh Kumar Tikoo Open Access Address Vaccine and Infectious Disease Organization University of Saskatchewan Saskatoon Saskatchewan Canada Email Yan Zhou - Andrew Ficzycz - aficzycz@ Suresh Kumar Tikoo - Corresponding author Published 12 June 2007 Received 5 April 2007 Accepted 12 June 2007 Virology Journal 2007 4 60 doi 1743-422X-4-60 This article is available from http content 4 1 60 2007 Zhou et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Replication-defective E1-E3 deleted adenovirus vector based gene delivery results in the induction of cytokines including IL-8 which may contribute to the development of inflammatory immune responses. Like other adenoviruses E1 E3 deleted porcine adenovirus PAdV 3 induces the production of IL-8 in infected cells. In contrast no IL-8 production could be detected in cells infected with wild-type or mutant PAdV-3s containing deletion in E1A E3 PAV211 or E1Bsmal E3 PAV212 . Expression of PAdV-3 E1 Blarge inhibited the NF-kB dependent transcription of luciferase from IL-8 promoter. Imunofluorescence and electrophoretic mobility shift assays suggested that constitutive expression of PAdV-3 E1Blarge inhibited the nuclear translocation of NF-kB and its subsequent binding to DNA. These results suggest that E1Blarge interacts with NF-kB to prevent transcription and down regulate proinflammatory cytokine IL-8 production. Background Cytokines are important mediators of inflammation and regulators of the immune response. The inflammatory response including release of
