Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition | Virology Journal BioMed Central Research Open Access Vaccinia virus AI2L protein and its AG A proteolysis play an important role in viral morphogenic transition Su Jung Yangt and Dennis E Hruby f Address Department of Microbiology Oregon State University Corvallis Oregon 97331-3804 USA Email Su Jung Yang - Dennis E Hruby - hrubyd@ Corresponding author fEqual contributors Published II July 2007 Received 29 June 2007 Accepted 11 July 2007 Virology Journal 2007 4 73 doi 1743-422X-4-73 This article is available from http content 4 1 73 2007 Yang and Hruby licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Like the major vaccinia virus VV core protein precursors p4b and p25K the 25 kDa VV A12L late gene product p 1 7K is proteolytically maturated at the conserved Ala-Gly-Ala motif. However the association of the precursor and its cleavage product with the core of mature virion suggests that both of the A12L proteins may be required for virus assembly. Here in order to test the requirement of the A12L protein and its proteolysis in viral replication a conditional lethal mutant virus wtetOA12L was constructed to regulate A12L expression by the presence or absence of an inducer tetracycline. In the absence of tetracycline replication of wtetOA12L was inhibited by 80 and this inhibition could be overcome by transient expression of the wild-type copy of the A12L gene. In contrast mutation of the AG A site abrogated the ability of the transfected A12L gene to rescue indicating that A12L proteolysis plays an important role in viral replication. Electron microscopy analysis of the A12L deficient virus demonstrated the aberrant virus particles which were displayed by .
