Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primer | Virology Journal BioMed Central Open Access Methodology Universal primers for HBV genome DNA amplification across subtypes a case study for designing more effective viral primers Qingrun Zhang11 Guanghua Wu11 Elliott Richards2 Shan gang Jia1 and Changqing Zeng 1 Address Beijing Institute of Genomics Chinese Academy of Sciences Beijing 101300 China and 2Department of Biology College of Biology and Agriculture Brigham Young University Provo UT 84602 USA Email Qingrun Zhang - zhangqr@ Guanghua Wu - wugh@ Elliott Richards - Shan gang Jia - jsg200830@ Changqing Zeng - czeng@ Corresponding author fEqual contributors Published 24 September 2007 Received 28 June 2007 Accepted 24 September 2007 Virology journal 2007 4 92 doi 1743-422X-4-92 This article is available from http content 4 1 92 2007 Zhang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The highly heterogenic characteristic of viruses is the major obstacle to efficient DNA amplification. Taking advantage of the large number of virus DNA sequences in public databases to select conserved sites for primer design is an optimal way to tackle the difficulties in virus genome amplification. Results Here we use hepatitis B virus as an example to introduce a simple and efficient way for virus primer design. Based on the alignment of HBV sequences in public databases and a program BxB in Perl script our method selected several optimal sites for HBV primer design. Polymerase chain reaction showed that compared with the success rate of the most popular primers for whole genome amplification of HBV one set of primers for full length genome amplification and four
