Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function | Olson et al. Journal of Translational Medicine 2011 9 26 http content 9 1 26 TRANSLATIONAL MEDICINE RESEARCH Open Access Shipping blood to a central laboratory in multicenter clinical trials effect of ambient temperature on specimen temperature and effects of temperature on mononuclear cell yield viability and immunologic function Walter C Olson1 Mark E Smolkin2 Erin M Farris3 Robyn J Fink4 Andrea R Czarkowski5 Jonathan H Fink6 Kimberly A Chianese-Bullock1 7 Craig L Slingluff Jr1 7 Abstract Background Clinical trials of immunologic therapies provide opportunities to study the cellular and molecular effects of those therapies and may permit identification of biomarkers of response. When the trials are performed at multiple centers transport and storage of clinical specimens become important variables that may affect lymphocyte viability and function in blood and tissue specimens. The effect of temperature during storage and shipment of peripheral blood on subsequent processing recovery and function of lymphocytes is understudied and represents the focus of this study. Methods Peripheral blood samples n 285 from patients enrolled in 2 clinical trials of a melanoma vaccine were shipped from clinical centers 250 or 1100 miles to a central laboratory at the sponsoring institution. The yield of peripheral blood mononuclear cells PBMC collected before and after cryostorage was correlated with temperatures encountered during shipment. Also to simulate shipping of whole blood heparinized blood from healthy donors was collected and stored at 15 C 22 C 30 C or 40 C for varied intervals before isolation of PBMC. Specimen integrity was assessed by measures of yield recovery viability and function of isolated lymphocytes. Several packaging systems were also evaluated during simulated shipping for the ability to maintain the internal temperature in adverse temperatures over time. Results Blood specimen containers experienced temperatures during .
