Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A novel multiplex assay combining autoantibodies plus PSA has potential implications for classification of prostate cancer from non-malignant cases | Xie et al. Journal of Translational Medicine 2011 9 43 http content 9 1 43 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access A novel multiplex assay combining autoantibodies plus PSA has potential implications for classification of prostate cancer from non-malignant cases Chong Xie1 Hyun J Kim2 Jonathan G Haw3 Anusha Kalbasi3 Brian K Gardner4 Gang Li5 Jianyu Rao6 David Chia6 l nnf I i inri7 Pl iHir i p Pl in 72 loir i8 I onntưrl Q K l2rlzc3 Ạ In I par ll i lz3 A ro Ho I2 Trillo9 1 ir imif i AÌ nn 1 Monty Liong Rubio R Punzalan Leonard S Marks Allan J Pantuck Alexandre de la Taille Guomin Wang Hideki Mukouyama10 and Gang Zeng3 Abstract Background The lack of sufficient specificity and sensitivity among conventional cancer biomarkers such as prostate specific antigen PSA for prostate cancer has been widely recognized after several decades of clinical implications. Autoantibodies autoAb among others are being extensively investigated as potential substitute markers but remain elusive. One major obstacle is the lack of a sensitive and multiplex approach for quantifying autoAb against a large panel of clinically relevant tumor-associated antigens TAA . Methods To circumvent preparation of phage lysates and purification of recombinant proteins we identified B cell epitopes from a number of previously defined prostate cancer-associated antigens PCAA . Peptide epitopes from cancer testis antigen NY-ESO-1 XAGE-1b SSX-2 4 as well as prostate cancer overexpressed antigen AMACR p90 autoantigen and LEDGF were then conjugated with seroMAP microspheres to allow multiplex measurement of autoAb present in serum samples. Moreover simultaneous quantification of autoAb plus total PSA was achieved in one reaction and termed the A PSA assay. Results Peptide epitopes from the above 6 PCAA were identified and confirmed that autoAb against these peptide epitopes reacted specifically with the full-length protein. A pilot study was conducted with the A PSA
