Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Pro-apoptotic activity of a-bisabolol in preclinical models of primary human acute leukemia cells | Cavalieri et al. Journal of Translational Medicine 2011 9 45 http content 9 1 45 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Pro-apoptotic activity of a-bisabolol in preclinical models of primary human acute leukemia cells Elisabetta Cavalieri1 Antonella Rigo2 Massimiliano Bonifacio2 Alessandra Carcereri de Prati1 Emanuele Guardalben2 Christian Bergamini3 Romana Fato3 Giovanni Pizzolo2 Hisanori Suzuki1 and Fabrizio Vinante2 Abstract Background We previously demonstrated that the plant-derived agent a-bisabolol enters cells via lipid rafts binds to the pro-apoptotic Bcl-2 family protein BID and may induce apoptosis. Here we studied the activity of a-bisabolol in acute leukemia cells. Methods We tested ex vivo blasts from 42 acute leukemias 14 Philadelphia-negative and 14 Philadelphia-positive B acute lymphoid leukemias Ph- Ph B-ALL 14 acute myeloid leukemias AML for their sensitivity to a-bisabolol in 24-hour dose-response assays. Concentrations and time were chosen based on CD34 CD33 my and normal peripheral blood cell sensitivity to increasing a-bisabolol concentrations for up to 120 hours. Results A clustering analysis of the sensitivity over 24 hours identified three clusters. Cluster 1 14 5 uM a-bisabolol IC50 included mainly Ph-B-ALL cells. AML cells were split into cluster 2 and 3 45 7 and 65 5 uM IC50 . Ph B-ALL cells were scattered but mainly grouped into cluster 2. All leukemias including 3 imatinib-resistant cases were eventually responsive but a subset of B-ALL cells was fairly sensitive to low a-bisabolol concentrations. a-bisabolol acted as a pro-apoptotic agent via a direct damage to mitochondrial integrity which was responsible for the decrease in NADH-supported state 3 respiration and the disruption of the mitochondrial membrane potential. Conclusion Our study provides the first evidence that a-bisabolol is a pro-apoptotic agent for primary human acute leukemia cells. Background a-bisabolol is a small oily .
