ATuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: dipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction | Chen et al. Journal of Translational Medicine 2011 9 51 http content 9 1 51 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction Yen-Ta Chen1t Cheuk-Kwan Sun2 10 Yu-Chun Lin3 4t Li-Teh Chang5 Yung-Lung Chen 3 Tzu-Hsien Tsai3 Sheng-Ying Chung3 Sarah Chua3 Ying-Hsien Kao6 Chia-Hung Yen7 Pei-Lin Shao8 Kuan-Cheng Chang9 Steve Leu3 4 and Hon-Kan Yip3 4 Abstract Background Reactive oxygen species are important mediators exerting toxic effects on various organs during ischemia-reperfusion IR injury. We hypothesized that adipose-derived mesenchymal stem cells ADMSCs protect the kidney against oxidative stress and inflammatory stimuli in rat during renal IR injury. Methods Adult male Sprague-Dawley SD rats n 24 were equally randomized into group 1 sham control group 2 IR plus culture medium only and group 3 IR plus immediate intra-renal administration of X 106 autologous ADMSCs followed by intravenous ADMSCs at 6 h and 24 h after IR . The duration of ischemia was 1 h followed by 72 hours of reperfusion before the animals were sacrificed. Results Serum creatinine and blood urea nitrogen levels and the degree of histological abnormalities were markedly lower in group 3 than in group 2 all p . The mRNA expressions of inflammatory oxidative stress and apoptotic biomarkers were lower whereas the anti-inflammatory anti-oxidative and anti-apoptotic biomarkers were higher in group 3 than in group 2 all p . Immunofluorescent staining showed a higher number of CD31 von Willebrand Factor and heme oxygenase HO -1 cells in group 3 than in group 2 all p . Western blot showed notably higher NAD P H quinone oxidoreductase 1 and HO-1 activities two indicators of anti-oxidative capacity in group 3 than those in group 2 all p . Immunohistochemical staining showed higher glutathione .
