Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Interferon signaling patterns in peripheral blood lymphocytes may predict clinical outcome after high-dose interferon therapy in melanoma patients | Simons et al. Journal of Translational Medicine 2011 9 52 http content 9 1 52 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Interferon signaling patterns in peripheral blood lymphocytes may predict clinical outcome after high-dose interferon therapy in melanoma patients Diana L Simons1 Gerald Lee1 John M Kirkwood2 and Peter P Lee1 Abstract Background High-dose Interferon HDI therapy produces a clinical response and achieves relapse-free survival in 20-33 of patients with operable high risk or metastatic melanoma. However patients may develop significant side effects frequently necessitating dose reduction or discontinuation of therapy. We recently showed that peripheral blood lymphocytes PBL from some melanoma patients have impaired interferon IFN signaling which could be restored with high concentrations of IFN. This exploratory study evaluated IFN signaling in PBL of melanoma patients to assess whether the restoration of PBL IFN signaling may predict a beneficial effect for HDI in melanoma patients. Methods PBL from 14 melanoma patients harvested on Day 0 and Day 29 of neoadjuvant HDI induction therapy were analyzed using phosflow to assess their interferon signaling patterns through IFN-a induced phosphorylation of STAT1-Y701. Results Patients who had a clinical response to HDI showed a lower PBL interferon signaling capacity than nonresponders at baseline Day 0 . Additionally clinical responders and patients with good long-term outcome showed a significant increase in their PBL interferon signaling from Day 0 to Day 29 compared to clinical nonresponders and patients that developed metastatic disease. The differences in STAT1 activation from pre- to post-HDI treatment could distinguish between patients who were inclined to have a favorable or unfavorable outcome. Conclusion While the sample size is small these results suggest that interferon signaling patterns in PBL correlate with clinical responses and may predict .
