Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Value of large scale expansion of tumor infiltrating lymphocytes in a compartmentalised gas-permeable bag: interests for adoptive immunotherapy | Zuliani et al. Journal of Translational Medicine 2011 9 63 http content 9 1 63 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Value of large scale expansion of tumor infiltrating lymphocytes in a compartmentalised gas-permeable bag interests for adoptive immunotherapy TEnrnoc 7i 11 i 2 n i1 3t II 11 ion F l ti ic13t C I Tiin Romo 1O2 1 M rio- bricfino DtinnlnlfìnnJ lc Hollo Rnnlnlo-Achior4 inumds zuiidni Julien David Syivdin Bercegedy MdHe dnisune rdiiuuiiiiiu Isdbeiie nuuue Astier Amir Khdmmdri2 Cécile Coissdc4 Bruno Delorme4 Sordyd Sd idgh1 dnd Brigitte Dréno1 2 3 Abstract Background Adoptive cell therdpy ACT hds emerged ds dn effective tredtment for pdtients with metdstdtic meldnomd. However there dre several logisticdl dnd sdfety concerns dssocidted with idrge-scdle ex vivo expdnsion of tumour-specific T lymphocytes for widespredd dVdildbility of ACT for cdncer pdtients. To dddress these problems we developed d specific compdrtmentdlised bdg dllowing efficient expdnsion of tumour-specific T lymphocytes in dn edsy hdndling closed system. Methods Stdrting from lymph nodes from eight meldnomd pdtients we performed d side-by-side compdrison of Tumour-Infiltrdting Lymphocytes TIL produced dfter expdnsion in the compdrtmentdlised bdg versus TIL produced using the stdnddrd process in pldtes. Proliferation yield vidbility phenotype dnd IFNg secretion were compdrdtively studied. Results We found no differences in proliferation yield dnd cell vidbility between both TIL production systems. Moreover edch of the cell products complied with our defined reledse criterid before being ddministered to the pdtient. The phenotype dndlysis indicdted thdt the compdrtmentdlised bdg fdvours the expdnsion of CD8 cells. Findlly we found thdt TIL stimuldted in bdgs were enriched in redctive CD8 T cells when co-cultured with the dutologous meldnomd cell line. Conclusions The stimuldtion of TIL with feeder cells in the specificdlly designed .
