Báo cáo sinh học: "Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication | Orlandi et al. Journal of Translational Medicine 2011 9 106 http content 9 1 106 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication Chiara Orlandi Greta Forlani Giovanna Tosi and Roberto S Accolla Abstract Background MHC class II transactivator CIITA inhibits the function of HTLV-2 Tax-2 viral transactivator and consequently the replication of the virus in infected cells. Moreover overexpression of the nuclear factor NF-YB that cooperates with CIITA for the expression of MHC class II genes results also in inhibition of Tax-2 transactivation. The purpose of this investigation was to assess the cellular and molecular basis of the CIITA-mediated inhibition on Tax-2 and the relative role of NF-YB in this phenomenon. Methods By co-immunoprecipitation of lysates from 293T cells cotransfected with CIITA or fragments of it and Tax-2 it was assessed whether the two factors interact in vivo. A similar approach was used to assess Tax-2-NF-YB interaction. In parallel deletion fragments of CIITA were tested for the inhibition of Tax-2-dependent HTLV-2 LTR-luciferase transactivation. Subcellular localization of CIITA and Tax-2 was investigated by immunofluorescence and confocal microscopy. Results CIITA and Tax-2 interact in vivo through at least two independent regions at the 1-252 N-term and at the 410-1130 C-term respectively. Interestingly only the 1-252 N-term region mediates Tax-2 functional inhibition. CIITA and Tax-2 are localized both in the cytoplasm and in the nucleus when separately expressed. Instead when coexpressed most of Tax-2 colocalize with CIITA in cytoplasm and around the nuclear membrane. The Tax-2 minor remaining nuclear portion also co-localizes with CIITA. Interestingly when CIITA nucleus-cytoplasm shuttling is blocked by leptomycin B treatment most of the Tax-2 molecules are

Không thể tạo bản xem trước, hãy bấm tải xuống
TÀI LIỆU LIÊN QUAN
TÀI LIỆU MỚI ĐĂNG
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.