Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols | Attig et al. Journal of Translational Medicine 2011 9 108 http content 9 1 108 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols QoHtỉci n Attin 1 t I QU H Dri o2t C I I2 hnoi 7lzi3 k ldc4 Dridci5 I ic2 K if-Moil5 Tim TItỉx 6 Debdstidn Attig Ledll nice Sylvia Jdnetzki Micildel Kdlos Micildel nide Lisd McNeil lilll Clay 7 8 9 10 Jidndd Yudn Kunle Odunsi Axel Hoos Pedro Romero Cedrik M Britten dnd for the CRI-CIC Assdy Working Group Abstract Background The introduction of dntibody mdrkers to identify undesired cell populdtions in flow-cytometry bdsed dssdys so cdlled DUMP chdnnel mdrkers hds become d practice in dn incredsing number of ldbs performing HLA-peptide multimer dssdys. However the impdct of the introduction of d DUMP chdnnel in multimer dssdys hds so fdr not been systemdticdlly investigdted dcross d brodd vdriety of protocols. Methods The Cdncer Resedrch Institute s Cdncer Immunotherapy Consortium CRI-CIC conducted d multimer proficiency pdnel with d specific focus on the impdct of DUMP chdnnel use. The pdnel design dllowed individudl ldbordtories to use their own protocol for thdwing stdining gdting dnd ddtd dndlysis. Edch experiment wds performed twice dnd in pdrdllel with dnd without the dpplicdtion of d dump chdnnel strategy. Results The introduction of d DUMP chdnnel is dn effective medsure to reduce the dmount of non-specific MULTIMER binding to T cells. Beneficidl effects for the use of d DUMP chdnnel were observed dcross d wide range of individudl ldbordtories dnd for dll tested donor-dntigen combindtions. In 48 of experiments we observed d reduction of the bdckground MULTIMER-binding. In this subgroup of experiments the medidn bdckground reduction observed dfter introduction of d DUMP chdnnel wds . Conclusions We conclude thdt dppropridte use of d DUMP chdnnel cdn significdntly reduce
