Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Susceptibility of different leukocyte cell types to Vaccinia virus infection | Virology Journal BioMed Central Research Open Access Susceptibility of different leukocyte cell types to Vaccinia virus infection Juana M Sanchez-Puig1 Laura Sanchez2 Garbine Roy2 and Rafael Blasco 1 Address 1Departamento de . Ctra. La Coruna km E-28040 Spain and 2Servicio de Inmunología. Hospital Ramón y Cajal. 28034 Madrid Spain Email Juana M Sanchez-Puig - spuig@ Laura Sanchez - Garbine Roy - Rafael Blasco - blasco@ Corresponding author Published 22 November 2004 Received II October 2004 Accepted 22 November 2004 Virology Journal 2004 1 10 doi I743-422X-I-I0 This article is available from http content I I I0 2004 Sánchez-Puig et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Vaccinia virus the prototype member of the family Poxviridae was used extensively in the past as the Smallpox vaccine and is currently considered as a candidate vector for new recombinant vaccines. Vaccinia virus has a wide host range and is known to infect cultures of a variety of cell lines of mammalian origin. However little is known about the virus tropism in human leukocyte populations. We report here that various cell types within leukocyte populations have widely different susceptibility to infection with vaccinia virus. Results We have investigated the ability of vaccinia virus to infect human PBLs by using virus recombinants expressing green fluorescent protein GFP and monoclonal antibodies specific for PBL subpopulations. Flow cytometry allowed the identification of infected cells within the PBL mixture I-5 hours after infection. Antibody labeling revealed that different cell populations had very .
