ETuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: ffect of oligonucleotide primers in determining viral variability within hosts | Virology Journal BioMed Central Methodology Open Access Effect of oligonucleotide primers in determining viral variability within hosts Maria Alma Bracho t Inmaculada Garcia-Robles Nuria Jimenez Manuela Torres-Puente Andrés Moya and Fernando González-Candelas Address Institut Cavanilles de Biodiversitat i Biologia Evolutiva Universitat de València Edifici Instituts Polígon La Coma s n Paterna València 46980 SPAIN Email Maria Alma Bracho - Inmaculada García-Robles - Nuria Jiménez - Manuela Torres-Puente - Andrés Moya - Fernando González-Candelas - Corresponding author tEqual contributors Published 09 December 2004 Received 22 October 2004 Accepted 09 December 2004 Virology Journal 2004 1 13 doi 1743-422X-1-13 This article is available from http content 1 1 13 2004 Bracho et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genetic variability in viral populations is usually estimated by means of polymerase chain reaction PCR based methods in which the relative abundance of each amplicon is assumed to be proportional to the frequency of the corresponding template in the initial sample. Although bias in template-to-product ratios has been described before its relevance in describing viral genetic variability at the intrapatient level has not been fully assessed yet. Results To investigate the role of oligonucleotide design in estimating viral variability within hosts genetic diversity in hepatitis C virus HCV populations from eight infected patients was characterised by two parallel PCR amplifications performed with two slightly different sets of primers .
