Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture | Virology Journal BioMed Central Research Open Access Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus analysis of consecutive passages in a cell culture Angelina Plyusnina and Alexander Plyusnin Address Haartman Institute Department of Virology University of Helsinki POB 21 FIN-00014 Helsinki Finland Email Angelina Plyusnina - Alexander Plyusnin - Corresponding author Published 22 February 2005 Received 01 February 2005 Accepted 22 February 2005 Virology Journal 2005 2 12 doi 1743-422X-2-12 This article is available from http content 2 1 12 2005 Plyusnina and Plyusnin licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Tula hantavirus carrying recombinant S RNA segment recTULV grew in a cell culture to the same titers as the original cell adapted variant but presented no real match to the parental virus. Our data showed that the lower competitiveness of recTULV could not be increased by pre-passaging in the cell culture. Nevertheless the recombinant virus was able to survive in the presence of the parental virus during five consecutive passages. The observed survival time seems to be sufficient for transmission of newly formed recombinant hantaviruses in nature. Background Recombination in RNA viruses serves two main purposes i it generates and spreads advantageous genetic combinations and ii it counters the deleterious effect of mutations that due to the low fidelity of viral RNA polymerases and lack of proofreading occur with high frequency 1 . The purging function is naturally attributed to the homologous recombination HRec . recombination between homologous parental
