Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protei | Virology Journal BioMed Central Short report Hepatitis B virus X protein interacts with p5 subunit of heterotrimeric guanine nucleotide binding protein Siew Hui Lwa and Wei Ning Chen Open Access Address School of Chemical and Biomedical Engineering and School of Biological Sciences College of Engineering Nanyang Technological University Blk 1 Innovation Centre 16 Nanyang Drive Unit 100 Level 1 Singapore 637722 Email Siew Hui Lwa - SHLwa@ Wei Ning Chen - WNChen@ Corresponding author Published 31 August 2005 Received 15 July 2005 Accepted 31 August 2005 Virology Journal 2005 2 76 doi 1743-422X-2-76 This article is available from http content 2 1 76 2005 Lwa and Chen licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background To isolate cellular proteins interacting with hepatitis B virus X protein HBX from HepG2 cells infected with hepatitis B virus HBV . Results HBV particles were produced in culture medium of HepG2 cells transfected with the mammalian expression vector containing the linear HBV genome as assessed by commercially available ELISA assay. A cDNA library was made from these cells exposed to HBV. From yeast two hybrid screening with HBX as bait human guanine nucleotide binding protein p subunit 5L GNp5 was isolated from the cDNA library constructed in this study as a new HBX-interacting protein. The HBX-GNP5 interaction was further supported by mammalian two hybrid assay. Conclusion The use of a cDNA library constructed from HBV-transfected HepG2 cells has resulted in the isolation of new cellular proteins interacting with HBX. Background Infection by hepatitis B virus HBV an enveloped DNA virus of the hepadnaviridae family has been closely related to .
