Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Platelet-activating factor enhancement of calcium influx and interleukin-6 expression, but not production, in human microglia | Journal of Neuroinflammation BioMed Central Research Open Access Platelet-activating factor enhancement of calcium influx and interleukin-6 expression but not production in human microglia Prasongchai Sattayaprasert11 3 Hyun B Chop1 2 Sukumal Chongthammakun3 and James G McLarnon 1 Address department of Pharmacology and Therapeutics Faculty of Medicine University of British Columbia Vancouver BC Canada division of Neurology Department of Medicine University of British Columbia Canada and department of Anatomy Mahidol University Bangkok Thailand Email Prasongchai Sattayaprasert - mannkung@ Hyun B Choi - chb1202@ Sukumal Chongthammakun - scsct@ James G McLarnon - mclarnon@ Corresponding author fEqual contributors Published 15 April 2005 Received 19 January 2005 Journal of Neuroinflammation 2005 2 11 doi 1742-2094-2-11 Accepted 15 Apnl 2005 This article is available from http content 2 1 11 2005 Sattayaprasert et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Calcium-sensitive fluorescence microscopy and molecular biology analysis have been used to study the effects of platelet-activating factor PAF on intracellular calcium Ca2 i and IL-6 expression in human microglia. PAF applied acutely at 100 nM elicited a biphasic response in Ca2 i consisting of an initial rapid increase of Ca2 i due to release from internal stores followed by a sustained influx. The latter phase of the Ca2 i increase was blocked by SKF96365 a non-selective store-operated channel SOC inhibitor. RT-PCR analysis showed PAF treatment of microglia induced expression of the pro-inflammatory cytokine IL-6 in a time-dependent manner which was blocked in the .
