Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Inhibitory effect of a tyrosine-fructose Maillard reaction product, 2,4-bis(p-hydroxyphenyl)-2butenal on amyloid-b generation and inflammatory reactions via inhibition of NF- B and STAT3 activation in cultured astrocytes and microglial BV-2 cells | Lee et al. Journal of Neuroinflammation 2011 8 132 http content 8 1 132 JJOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Inhibitory effect of a tyrosine-fructose Maillard reaction product 2 4-bis p-hydroxyphenyl -2-butenal on amyloid-p generation and inflammatory reactions via inhibition of NF- .-B and STAT3 activation in cultured astrocytes and microglial BV-2 cells w Z-I 1 4 Young-Jung Lee Dong-Young Choi Im Seup Choi Jin-Yi Han Heon-Sang Jeong Sang Bae Han Ki-Wan Oh1 and Jin Tae Hong1 2 3 Abstract Background Amyloidogenesis is linked to neuroinflammation. The tyrosine-fructose Maillard reaction product 2 4-bis p-hydroxyphenyl -2-butenal possesses anti-inflammatory properties in cultured macrophages and in an arthritis animal model. Because astrocytes and microglia are responsible for amyloidogenesis and inflammatory reactions in the brain we investigated the anti-inflammatory and anti-amyloidogenic effects of 2 4-bis p-hydroxyphenyl -2-butenal in lipopolysaccharide LPS -stimulated astrocytes and microglial BV-2 cells. Methods Cultured astrocytes and microglial BV-2 cells were treated with LPS 1 pg ml for 24 h in the presence 1 2 5 pM or absence of 2 4-bis p-hydroxyphenyl -2-butenal and harvested. We performed molecular biological analyses to determine the levels of inflammatory and amyloid-related proteins and molecules cytokines Ap and secretases activity. Nuclear factor-kappa B NF-kB DNA binding activity was determined using gel mobility shift assays. Results We found that 2 4-bis p-hydroxyphenyl -2-butenal 1 2 5 pM suppresses the expression of inducible nitric oxide synthase iNOS and cyclooxygenase-2 COX-2 as well as the production of nitric oxide NO reactive oxygen species ROS tumor necrosis factor-a TNF-a and interleukin-1p IL-1P in LPS 1 pg ml -stimulated astrocytes and microglial BV-2 cells. Further 2 4-bis p-hydroxyphenyl -2-butenal inhibited the transcriptional and DNA binding activity of NF-KB-a .
