Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Origin and consequences of brain Toll-like receptor 4 pathway stimulation in an experimental model of depression | Gárate et al. Journal of Neuroinflammation 2011 8 151 http content 8 1 151 JIOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Origin and consequences of brain Toll-like receptor 4 pathway stimulation in an experimental model of depression Iz-i- r cz A z Fz 1 4 5 D f z I vr Oi I f 1 4 5 I f r-I k K h K z l ri Z i s I 1 4 5 I I zd I o z-sx z 3 4 c r 1 rw Oz vvr r r r z 3 4 I r l zxr D X r z 2 4 5 iciar Gárate Borja Garcia-Bueno Jose LM Maarigai Lidia Bravo Esther Berrocoso Javier R Caso Juan A Micó3 4 and Juan C Leza1 4 5 Abstract Background There is a pressing need to identify novel pathophysiological pathways relevant to depression that can help to reveal targets for the development of new medications. Toll-like receptor 4 TLR-4 has a regulatory role in the brain s response to stress. Psychological stress may compromise the intestinal barrier and increased gastrointestinal permeability with translocation of lipopolysaccharide LPS from Gram-negative bacteria may play a role in the pathophysiology of major depression. Methods Adult male Sprague-Dawley rats were subjected to chronic mild stress CMS or CMS intestinal antibiotic decontamination CMS ATB protocols. Levels of components of the TLR-4 signaling pathway of LPS and of different inflammatory oxidative nitrosative and anti-inflammatory mediators were measured by RT-PCR western blot and or ELISA in brain prefrontal cortex. Behavioral despair was studied using Porsolt s test. Results CMS increased levels of TLR-4 and its co-receptor MD-2 in brain as well as LPS and LPS-binding protein in plasma. In addition CMS also increased interleukin IL -ip COX-2 PGE2 and lipid peroxidation levels and reduced levels of the anti-inflammatory prostaglandin 15d-PGJ2 in brain tissue. Intestinal decontamination reduced brain levels of the pro-inflammatory parameters and increased 15d-PGJ2 however this did not affect depressive-like behavior induced by CMS. Conclusions Our results suggest that LPS from .
