Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Pilose antler polypeptides promote chondrocyte proliferation via the tyrosine kinase signaling pathway | Lin et al. Journal of Occupational Medicine and Toxicology 2011 6 27 http content 6 1 27 _ JOURNAL OF OCCUPATIONAL MEDICINE AND TOXICOLOGY RESEARCH Open Access Pilose antler polypeptides promote chondrocyte proliferation via the tyrosine kinase signaling pathway Jian-Hua Lin1 Ling-Xiao Deng1 Zhao-Yang Wu1 Lei Chen1 and Li Zhang2 Abstract Background Pilose antler polypeptides PAP have been reported to promote chondrocyte proliferation. However the underlying mechanism remains unclear. The present study was to investigate the effects of PAP on the proliferation of chondrocytes and its underlying mechanism. Methods Chondrocytes isolated from the knee of Zealand white rabbits were cultured. The second generation chondrocytes were collected and identified using safranin-O staining. The chondrocytes were divided into the following 4 groups including serum-free PAP genistein an inhibitor of tyrosine kinases and PAP plus genistein group. Cell viability was analyzed using the MTT assay. The cell cycle distribution of the chondrocytes was analyzed by flow cytometry. The expression levels of cyclin A was detected using immunocytochemical staining. Results No significant difference was observed between serum-free and genistein group. Treatment of the cultures with PAP produced a significant dose-dependent increase in cell viability the percentage proportion of chondrocytes in the S phase and Cyclin A expression as well. However the promoting effect of PAP on chondrocyte proliferation were dose-dependently inhibited by genistein whereas genistein alone had no effect on proliferation of isolated chondrocytes. Conclusions The data demonstrate that PAP promotes chondrocyte proliferation with the increased cell number percentage proportion of chondrocytes in S phase and expression of protein cyclin A via the TK signaling pathway. Keywords PAP Chondrocyte Proliferation S phase Cyclin A TK signaling pathway Introduction The facts that cartilage in deer antler grows
