Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations | Journal of Orthopaedic Surgery and Research BioMed Central Research article Effects of TGF-P1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations Rongfeng Zhang Dike Ruant and Chao Zhangt Open Access Address Department of Orthopaedic Surgery Navy General Hospital of PLA Beijing 100037 People s Republic of China Email Rongfeng Zhang - zhangrongfeng200@ Dike Ruan - ruandike@ Chao Zhang - zcmail2002@ Corresponding author fEqual contributors Published 26 October 2006 Received 23 February 2006 Journal of Orthopaedic Surgery and Research 2006 1 9 doi 1749-799X-1-9 Accepted 26 October 2006 This article is available from http content 1 1 9 2006 Zhang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The low proliferative viability of human nucleus pulposus NP cells is considered as a cause of intervertebral discs degeneration. Growth factors such as TGF-P1 and IGF-1 have been implicated in cell proliferation and matrix synthesis. Objective To investigate the dose-response and time-course effect of transforming growth factorP1 TGF-P1 and insulin-like growth factor-1 IGF-1 on proliferation of NP cells. Study design 3- 4 5-dimethylthiazolyl -2 5-diphenyl-tetrazolium bromide MTT is reduced by dehydrogenase in mitochondria of live cells. The proliferative viability of cells corresponds to the amount of MTT reduced which is measured with an enzyme-linked immunosorbent assay plate reader. In this study we assessed dose- and time-dependent effects of NP cells to TGF-P1 and IGF-1 in medium with different serum concentrations by MTT assay. Methods After release of informed consent tissue samples of NP were .
