Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Differential expression of type X collagen in a mechanically active 3-D chondrocyte culture system: a quantitative study | BioMed Central Journal of Orthopaedic Surgery and Research Research article Differential expression of type X collagen in a mechanically active 3-D chondrocyte culture system a quantitative study Xu Yangt Peter S Vezeridist Brian Nicholas Joseph J Crisco Douglas C Moore and Qian Chen Open Access Address Orthopaedic Research Laboratories Department of Orthopaedics Brown Medical School Rhode Island Hospital Providence RI 02903 USA Email Xu Yang - xu_yang@ Peter S Vezeridis - peter_vezeridis@ Brian Nicholas - bwnicholas@ Joseph J Crisco - Joseph_Crisco@ Douglas C Moore - Douglas_Moore@ Qian Chen - Qian_Chen@ Corresponding author fEqual contributors Published 06 December 2006 Received 07 March 2006 __J . r I I ir Accepted 06 December 2006 Journal of Orthopaedic Surgery and Research 2006 1 15 doi 1749-799X-1-15 This article is available from http content 1 1 15 2006 Yang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Objective Mechanical loading of cartilage influences chondrocyte metabolism and gene expression. The gene encoding type X collagen is expressed specifically by hypertrophic chondrocytes and up regulated during osteoarthritis. In this study we tested the hypothesis that the mechanical microenvironment resulting from higher levels of local strain in a three dimensional cell culture construct would lead to an increase in the expression of type X collagen mRNA by chondrocytes in those areas. Methods Hypertrophic chondrocytes were isolated from embryonic chick sterna and seeded onto rectangular Gelfoam sponges. Seeded sponges were subjected to various levels of cyclic uniaxial tensile strains at 1 Hz with the .
