Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Tissue specific characteristics of cells isolated from human and rat tendons and ligaments | Journal of Orthopaedic Surgery and Research BioMed Central Open Access Research article Tissue specific characteristics of cells isolated from human and rat tendons and ligaments N Scutt1 CG Rolf2 and A Scutt 3 Address Dept. of Engineering Materials Sir Robert Hadfield Building Mappin Street Sheffield S1 3JD UK 2Sheffield Centre of Sports Medicine 5 Broomfield Road Sheffield S10 2SE UK and 3Section of Musculoskeletal Science School of Medicine and Biomedical Sciences University of Sheffield Beech Hill Road Sheffield S10 2RX UK Email N CG Rolf - christerrolf@ AScutt - Corresponding author Published 24 July 2008 Received 31 December 2007 Journal of Orthopaedic Surgery and Research 2008 3 32 doi 1749-799X-3-32 Accepted 24 July 2008 This article is available from http content 3 1 32 2008 Scutt et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Tendon and ligament injuries are common and costly in terms of surgery and rehabilitation. This might be improved by using tissue engineered constructs to accelerate the repair process a method used successfully for skin wound healing and cartilage repair. Progress in this field has however been limited possibly due to an over-simplistic choice of donor cell. For tissue engineering purposes it is often assumed that all tendon and ligament cells are similar despite their differing roles and biomechanics. To clarify this we have characterised cells from various tendons and ligaments of human and rat origin in terms of proliferation response to dexamethasone and cell surface marker expression. Methods Cells isolated from tendons by collagenase digestion were plated out in DMEM .
