Tham khảo tài liệu 'báo cáo hóa học: " hiv-1 vpr activates the g2 checkpoint through manipulation of the ubiquitin proteasome system"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Open Access HIV-I Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system Jason L DeHart 1 Erik S Zimmerman 1 Orly Ardon1 Carlos MR Monteiro- Filho2 Enrique RArganaraz2 and Vicente Planelles 1 Address 1Division of Cell Biology and Immunology Department of Pathology University of Utah School of Medicine 15 North Medical Drive East 2100 - Room 2520 Salt Lake City UT 84112 USA and 2Laboratório de Farmacologia Molecular CP 04536 Faculdade de Saude Universidade de Brasilia 70919-970 Brasilia DF Brazil Email Jason L DeHart - Erik S Zimmerman - Orly Ardon - Carlos MR Monteiro-Filho - carlosmonteirofilho@ Enrique R Arganaraz - enrique@ Vicente Planelles - Corresponding author fEqual contributors Published 8 June 2007 Received 4 June 2007 Accepted 8 June 2007 Virology Journal 2007 4 57 doi l 743-422X-4-57 This article is available from http content 4 1 57 2007 DeHart et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract HIV-1 Vpr is a viral accessory protein that activates ATR through the induction of DNA replication stress. ATR activation results in cell cycle arrest in G2 and induction of apoptosis. In the present study we investigate the role of the ubiquitin proteasome system UPS in the above activity of Vpr. We report that the general function of the UPS is required for Vpr to induce G2 checkpoint activation as incubation of Vpr-expressing cells with proteasome inhibitors abolishes this effect. We further investigated in detail the specific E3 ubiquitin ligase subunits that Vpr manipulates. We
